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. 2014 Jul 11;16(6):811–816. doi: 10.4103/1008-682X.132471

Figure 5.

Figure 5

Hormonal regulation of 11β-HSD1 protein levels. The samples were from rats treated with vehicle (CON), NalGlu (NG), NalGlu plus testosterone (NG + T), NalGlu plus LH (NG + LH), or NalGlu plus MENT (NG + MENT). (a) Shows the protein levels of 11β-HSD1 relative to β-actin at PND 21, and (b) shows the protein levels of 11β-HSD1 relative to β-actin at PND 90 (means ± s.e.m., n = 3–6). The superscript asterisks show the statistical difference between two groups (**P < 0.01, one-way ANOVA with Tukey's post-hoc analysis). (c) Shows the pattern of immunoreactive proteins recognized by the specific antibodies. C1: 11β-HSD1 of PND 90 at 34 kDa; C2: β-actin of PND 90 at 42 kDa; C3: 11β-HSD1 of PND 21 at 34 kDa; C4: β-actin of PND21 at 42 kDa. PND: postnatal day; 11β-HSD1: 11β-hydroxysteroid dehydrogenase type 1; 11β-HSD2: 11β-hydroxysteroid dehydrogenase type 2; CON: control; NalGlu: [Ac-D2Nal1, 4C1DPhe2, D3Pal3, Arg5, DGlu6 (anisole adduct), DAla10]-GnRH; T: testosterone; LH: luteinizing hormone; MENT: 7α-methyl-nortestosterone; s.e.m.: standard error of the mean.