Figure 2.

Eugenol exposure has significant effects on three ORs in S100a5–tauGFP mice. A, The distribution of OR mRNAs between GFP⁺ and GFP⁻ samples is consistent across experiments. GFP⁺/GFP⁻ ratios from mice exposed to vehicle (mineral oil) from four replications, distinguished by color (red, blue, green, and black), plotted against the mean GFP⁺/GFP⁻ ratios of these four vehicle treatments. B, Mean OR mRNA GFP⁺/GFP⁻ ratio values after exposure to eugenol plotted against the mean ratios after exposure to vehicle (n = 4 groups of mice). Three ORs (Olfr961, Olfr958, and Olfr960) show significant elevation (FDR < 0.10) after eugenol exposure. To illustrate the change, their GFP⁺/GFP⁻ ratio values in this experiment (green diamonds) are compared with the GFP⁺/GFP⁻ ratio coordinates in other experiments that did not use eugenol (blue circles). C, Chemical structure of eugenol. D, Mean GFP⁺/GFP⁻ ratios and corresponding FDR probabilities for the seven ORs with FDR probabilities <0.5.