Figure 11.

TRPV4 regulates GFAP expression in Müller glia. A, Two weeks after a single intravitreal injection of a vehicle or 2 μl of 75 μm GSK101, mouse eyes were harvested and processed for IHC. Only astrocytes on the vitreal surface expressed GFAP in control eyes, whereas Müller glia also expressed GFAP in the GSK101-injected eyes of WT mice. TRPV4^−/− Müller glia were reactive in both conditions, but GSK101 did not further increase gliosis. B, The GFAP optical density was significantly greater in the GSK101-injected eyes. n = 3 for each. Scale bar, 20 μm. OLM, Outer limiting membrane; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; ILM, inner limiting membrane; a.u., arbitrary units. *p < 0.05. **p < 0.01.