TRPV4 mediates responses to GSK101 in Müller cells. A, B, External calcium is required for GSK101-induced calcium elevations. n = 16. C, D, Responses to GSK101 persist in the presence of the voltage-gated channel blocker cadmium (Cd2+). n = 4. E, F, The nonselective TRP channel antagonist Ruthenium Red (RuR) blocks responses to GSK101. n = 21 for GSK101; n = 15 for GSK101 + RuR. G, H, GSK101 responses are also blocked by the selective TRPV4 antagonist HC-06. This inhibition is reversible following a washout. n = 30, except noise = 40. **p < 0.01. ****p < 0.0001.