TRPV4 mediates RGC and Müller cell responses to HTS and GSK101. A, The percentage of RGCs responding to 50% HTS and 25 nm GSK101 is reduced by TRPV4 ablation (n = 4–9 experiments, two-way ANOVA). B, Calcium elevations induced by HTS and GSK are smaller in TRPV1−/− RGCs (N = 12–74 cells, two-way ANOVA). C, The percentage of Müller cells responding to 50% HTS and 25 nm GSK101 is reduced by TRPV4 ablation (n = 3–8 experiments, two-way ANOVA). D, Calcium elevations induced by HTS and GSK are smaller in TRPV1−/− Müller glia (n = 14–66 cells, two-way ANOVA). *p < 0.05. **p < 0.01. ***p < 0.001. ****p < 0.0001.