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. 2014 Nov 19;34(47):15764–15778. doi: 10.1523/JNEUROSCI.2267-14.2014

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Copyright © 2014 the authors 0270-6474/14/3415764-15$15.00/0

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Figure 10. — Myelin deficit in the spinal cord of Rheb1^f/f;Olig1^Cre+/− and Rheb1^f/f;CNP^Cre+/− mice. A, B, Black Gold staining of P28 control (Rheb1^f/+;Olig1^Cre+/−) and Rheb1^f/f; Olig1^Cre+/− (A) and control (Rheb1^f/+;CNP^Cre+/−) and Rheb1^f/f; CNP^Cre+/− (B) mice. Arrows indicate the Black Gold-positive areas in control and comparable areas in mutant brain. Scale bar, 200 μm. Insets show higher magnification of the boxed regions. Scale bar, 100 μm. C, D, Western blot analysis of myelin protein expression in the spinal cord (SC) of Rheb1^f/f; Olig1^Cre+/− (C) and Rheb1^f/f; CNP^Cre+/− mice (D) at P14 and P28. All quantifications are expressed as percentage control ± SEM. C, P14: MBP, **p = 0.0096; MOG,*p = 0.0132; CNP, *p = 0.0356; P28: MBP, **p = 0.0049; MOG, **p = 0.0055; CNP, **p = 0.0022. D, P14: MBP, ***p = 0.0003; MOG,*p = 0.0164; CNP, **p = 0.0020; P28: MBP, **p = 0.0029; MOG, ***p = 0.0004; CNP, *p = 0.0170. E, F, Immunostaining of CC1+/Olig2+ cells (arrows) in the spinal cord of Rheb1^f/f;Olig1^Cre+/− (E) and Rheb1^f/f;CNP^Cre+/− (F) mutants at P14 and P28. Quantification of total Olig2+ (OL lineage cells) and CC1+/Olig2+ (differentiated OLs) in the spinal cord are shown (bottom). E, P14, *p = 0.0254; P28,*p = 0.0102; (F) P14, *p = 0.0271. Scale bar, 40 μm.