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. 2014 Nov 14;12:71. doi: 10.1186/s12964-014-0071-9

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© Li et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Akt phosphorylation in HepG2 cells overexpressing Nck. (A) Total cell lysates from HepG2 cells transfected with control or Nck1 siRNA were subjected to immunoblot with indicated antibodies. Bar charts represent mean ± SEM of the ratio of Nck2/β-Actin. **P <0.01 versus control. Total cell lysates from HepG2 cells transfected with pRK5, HA-Nck2 (B) or HA-Nck1 (C) plasmids were subjected to immunoblot with indicated antibodies. Data are representative of three independent experiments showing similar results.