Figure 1.

Proportion of CD4 ⁺ T-cell subsets infected by Env-pseudotyped GFP reporter viruses. (a) Values represent the percentage of infected CD4⁺ T-cells that belong to the indicated subset; naïve (T_N, dark blue), stem cell memory (T_SCM, red), central memory (T_CM, yellow), transitional memory (T_TM, light blue), effector memory (T_EM, purple) and effector memory RA (T_EMRA, green). Values were averaged across four HIV-seronegative donors. Error bars represent the standard deviation. CD4⁺ T-cells were infected with GFP reporter viruses pseudotyped with (i) subtype C HIV-1 Envs isolated from plasma samples of subjects 1503, 1854 and 1109 provided at enrolment (E), 1-year later (I; intermediate) and 3-years after enrolment (F; final), or (ii) subtype B HIV-1 control Envs. Text in parentheses indicates the number of Envs isolated per plasma sample and/or Env coreceptor usage; CCR5-specific (R5), CXCR4-specific (X4), uses CCR5 or CXCR4 (R5X4). (b) Values represent the percentage of infected CD4⁺ T-cells that are T_SCM for each Env-pseudotyped GFP reporter virus per donor (individual dots). Columns display the mean and standard deviation. Statistical analyses were performed using Mann Whitney tests, p-values ≤0.05 were considered significant.