Table 1.

Subject 1503, 1854 and 1109 Envs

Subject	Plasma sample	CD4 + T-cell count (cells/μL)	Viral Load (RNA copies/ml)	Env ID	Coreceptor usage
1503	E	396	5.73	11	R5
				13	R5
	I	168	5.69	7	R5
				11	R5
	F	259	5.71	7	R5
				8	R5
1854	E	435	5.61	8	R5
				10	R5
	I	279	6.57	4	R5
				20	R5
	F	272	5.67	4	R5
				8	R5
1109	E	367	5.54	10	R5
				42	R5
	I	402	5.59	3	R5
	F	169	5.67	30	X4
				39	X4
	-	-	-	F-30-E10V3	R5

The CD4⁺ T-cell counts and plasma viral loads for these subjects have been reported previously, and are summarized again here to assist in the interpretation of the CD4⁺ T-cell subset tropism data [12]. Plasma viral load values are shown as log₁₀. Envs were cloned directly from plasma samples provided at enrolment (E), 1-year later (I; intermediate) and 3-years after enrolment (F; final). Env coreceptor usage was determined by infecting NP2 or U87 cells expressing CD4 and either CCR5 or CXCR4 with Env-pseudotyped luciferase reporter viruses [12]. The 1109-F-30-E10V3 Env was generated by mutating 1109-F-30 to express the V3 region of 1109-E-10 [12,17]. Env ID, HIV-1 Env identification number as described in [12].