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. 2014 Nov 8;15:371. doi: 10.1186/1471-2474-15-371

Table 1.

PCR primer sequences for COL9A1, COL9A2, COL9A3, COMP, and MATN3

Primer sequences
*COL9A1* gene		Tm (°C)	Product size (bp)
Exon 8-10	F - CCGATGTGCTCCACTAACCT	56	824
	R - GGCCAAGTTTAGAGCCACAG	56	824
COL9A2 gene
Exon 2	F - CAGCTTCCTGCACTGTCTGA	56	241
	R - GACGAGGGGCACTACATCTC	56	241
Exon 3-4	F - TGAGCCGTAGTGTGCTGTCT	56	278
	R - CTGGAGGTCAATTGGCAGAG	56	278
COL9A3 gene
Exon 2	F - TTTGGGTCTCACCGAGGA	56	293
	R - GCCTGGTTTTCTCTCCATCA	56	293
Exon 3	F - CTTGAGGGACCCCTGATTTT	56	152
	R - TGTTCTGAGTTCCCCCTTTC	56	152
Exon 4	F - GCATTTTGCTTCATTGCTGA	56	222
	R - AATTAGGGCCGGACTCCTC	56	222
COMP gene
Exon 8-9	F - TTGAGGCGGGGTTGGGTG	64	413
	R - ACCGTGCCGAGCCGTAGAT	64	413
Exon 10	F - AGGAGTGTGACCTTTGCCTTCT	64	334
	R - CTAGTCCAGCTTACCCCATCC	64	334
Exon 11-12	F - GAAGTCATTCTGGCCTGGTC	64	518
	R - AGCGTTTTGTCAAAGGCTACC	64	518
Exon 13	F - CGGGTAGCCTTTGACAAAACG	62	331
	R - GCCCGCCCACCGTAGAC	62	331
Exon 14-15	F - GGCGGGCCCTGACTTTAG	64	546
	R - ATAACCCCGCCCCTCTGT	64	546
Exon 16	F - GTTCTGGGTGCCAGGTTC	64	335
	R - AAGGGTTTTACGGAGGGTCAT	64	335
Exon 17	F - TGCTCCCAACTGTCTCTCCA	64	312
	R - ACCTGGGCCTGTGTGTCC	64	312
Exon 18-19	F - TCTGAGAGGGAAGGGTCTGG	64	443
	R - CCCTTCTCACTTCCCCCTCA	64	443
MATN3 gene
Exon 3	F - AAAGGAGCCCAGAGAGCAAT	59	290
	R - CAGTCCAAAACCTGGAGCAT