Table 1.
Aromatic/arginine selectivity filter residues and substitutions in the conserved NPA motifs in different fungal MIP subgroups
| |
|
Aromatic/arginine selectivity filter
c
|
|
|||
|---|---|---|---|---|---|---|
| Fungal MIP subgroup a | Number of sequences/Total number of sequences b | TM2 | TM5 | LE1 | LE2 | NPA substitution (%) d |
| FPS-1 like AQGPs |
25/26 |
W |
L/I/M/N |
T/C/A/S |
R |
61.7 |
| Facultative |
17/42 |
W |
A/S/T/G/D |
G/A |
R |
66.7 |
| (Yfl054-like) |
8/42 |
W |
V/I |
G |
R |
|
| AQGPs |
5/42 |
W |
M |
L |
R |
|
| |
8/42 |
W |
G |
F/Y |
R |
|
| α-cluster AQGPs |
46/48 |
W |
G |
Y/W/F |
R |
95.8 |
| β-cluster AQGPs |
20/24 |
W |
G |
Y |
R |
12.5 |
| γ1-cluster AQGPs |
4/5 |
T/N/S |
C/T |
F |
R |
20 |
| γ2-cluster AQGPs |
5/5 |
W/M/I |
G |
Y |
R |
0 |
| δ-cluster AQGPs |
49/49 |
F/Y/M |
V/I/A |
I/L/V |
R |
100 |
| AQPs |
76/163 |
F/M/A |
H |
T/A/C/S |
R |
|
| |
64/163 |
F/I/A |
E |
G |
R |
28.8 |
| |
15/163 |
Y/F |
M/L/V/A |
A/G |
R |
|
| |
8/163 |
F/M |
Q |
C/G |
R |
|
| XIPs |
17/17 |
N/S/A |
S/A/G |
A/F |
R/K |
82.4 |
| SIP-like | 16/16 | V/I/L/M | A/S/G | G | L/I | 81.2 |
aFor details of different fungal MIP subgroups, see Figure 1 and the text.
bNumber of sequences possessing a particular type of Ar/R selectivity filter residues and the total number of sequences belonging to that particular fugal MIP subgroup are given.
cThe four residues forming the selectivity filter of an MIP channel are contributed by the second (TM2) and fifth (TM5) transmembrane segments and two residues from the loop E region (LE1 and LE2).
dPercentage of sequences from that particular fungal MIP subgroup that has substitution(s) in at least one of the conserved NPA motifs.