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Purification steps of the enzyme chitinase from Bacillus subtilis TV-125A
| Purification steps |
Volume |
Activity |
Total activity |
Protein amount |
Specific activity |
Purificationfold | |
|---|---|---|---|---|---|---|---|
| (mL) | (EU/mL) | EU | % | (mg/mL) | (EU/mg) | ||
|
Crude extrat |
50 |
44.95 |
8155.0 |
100 |
6.46 |
6.95 |
- |
|
Ammonium sulphate precipitation |
5 |
255.5 |
1277.5 |
15.7 |
1.64 |
155.79 |
22.4 |
|
(%0-20) | |||||||
| DEAE Sephadex column | 20 | 55.2 | 1104.0 | 13.4 | 0.28 | 197.14 | 28.4 |
