Table 2.
Fold change in gene expression along the central metabolism and mixed-acid fermentation pathways
|
Gene |
Product |
PM vs. WT 0 |
PM vs. WT 10 |
PM 0 vs. 10 |
PM 0 vs. 17.5 |
WT 0 vs. 10 |
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| ML | LL | ML | LL | ML | LL | ML | LL | ML | LL | ||
| glucose-6-phosphate to PEP | |||||||||||
| Cthe_0347 |
Phosphofructokinase |
1.77 |
2.59 |
2.97 |
2.13 |
−1.35 |
−1.31 |
−1.14 |
−1.49 |
−2.27 |
−1.07 |
| Cthe_0349 |
fructose-1,6-bisphosphate aldolase, class II |
1.60 |
2.49 |
3.31 |
2.50 |
−1.52 |
−1.41 |
−1.49 |
−2.03 |
−3.14 |
−1.42 |
| Cthe_2449 |
Phosphoglycerate mutase |
−2.46 |
−1.85 |
1.42 |
−1.74 |
−1.48 |
−1.90 |
−2.01 |
−2.88 |
−5.18 |
−2.03 |
| Cthe_3153 |
alpha-ribazole phosphatase |
2.11 |
2.33 |
1.40 |
1.40 |
1.21 |
1.23 |
1.42 |
−1.14 |
1.82 |
2.04 |
| Cthe_0143 |
Enolase |
−1.23 |
−1.04 |
1.63 |
−1.02 |
−1.11 |
−1.13 |
−1.05 |
−2.96 |
−2.22 |
−1.16 |
| Non-oxidative Pentose Phosphate pathway | |||||||||||
| Cthe_2443 |
Transketolase domain-containing protein |
−3.24 |
−4.70 |
1.02 |
−3.00 |
1.14 |
−1.75 |
−1.90 |
−1.52 |
−2.88 |
−2.74 |
| Cthe_2444 |
Transketolase domain-containing protein |
−3.47 |
−4.63 |
−1.15 |
−3.39 |
1.26 |
−1.72 |
−1.63 |
−1.57 |
−2.41 |
−2.36 |
| Cthe_2705 |
Transketolase central region |
−1.44 |
−1.33 |
2.25 |
1.19 |
1.24 |
1.21 |
1.17 |
−1.81 |
−2.60 |
−1.32 |
| PEP to Pyruvate | |||||||||||
| Cthe_2874 |
Phosphoenolpyruvate carboxykinase [GTP] |
1.39 |
2.46 |
1.43 |
2.09 |
−1.05 |
−1.04 |
1.30 |
1.38 |
−1.07 |
1.14 |
| Cthe_0344 |
malic protein NAD-binding |
−1.68 |
1.06 |
1.26 |
−1.10 |
−1.01 |
−1.14 |
1.20 |
−1.27 |
−2.13 |
1.02 |
| Cthe_1308 |
pyruvate, phosphate dikinase |
1.64 |
2.29 |
−1.30 |
1.65 |
−1.05 |
1.07 |
1.30 |
1.10 |
2.03 |
1.49 |
| Pyruvate to Lactate/Formate/Acetyl-CoA | |||||||||||
| Cthe_1053 |
L-lactate dehydrogenase |
−1.78 |
−1.25 |
1.32 |
−1.02 |
−1.41 |
−1.27 |
−1.33 |
−1.16 |
−3.30 |
−1.55 |
| Cthe_2794 |
pyruvate/ketoisovalerate oxidoreductase, gamma subunit |
4.30 |
1.48 |
5.15 |
3.99 |
1.92 |
2.56 |
2.45 |
2.78 |
1.61 |
−1.05 |
| Cthe_2796 |
pyruvate flavodoxin/ferredoxin oxidoreductase domain protein |
3.13 |
1.47 |
4.16 |
2.94 |
1.98 |
2.05 |
1.88 |
1.94 |
1.49 |
1.02 |
| Cthe_0505 |
formate acetyltransferase |
−1.95 |
−1.91 |
1.46 |
−1.04 |
1.24 |
1.04 |
1.11 |
−1.81 |
−2.31 |
−1.76 |
| Acetyl-CoA to Ethanol/Acetate | |||||||||||
| Cthe_1028 |
Acetate kinase |
1.67 |
2.57 |
3.63 |
3.05 |
2.12 |
1.26 |
2.76 |
1.50 |
−1.02 |
1.06 |
| Cthe_1029 |
phosphate acetyltransferase |
1.54 |
1.79 |
4.01 |
3.83 |
2.42 |
1.33 |
2.73 |
1.63 |
−1.08 |
−1.61 |
| Cthe_2238 |
Aldehyde Dehydrogenase |
1.06 |
1.04 |
−1.81 |
−1.29 |
1.20 |
1.36 |
1.36 |
1.02 |
2.30 |
1.83 |
| Cthe_0101 |
iron-containing alcohol dehydrogenase |
−1.35 |
−1.19 |
2.19 |
1.20 |
1.22 |
−1.04 |
−1.18 |
−1.82 |
−2.43 |
−1.48 |
| Cthe_0423 | iron-containing alcohol dehydrogenase | 1.12 | 1.07 | 4.75 | 5.02 | 1.26 | 1.06 | 1.28 | 1.45 | −3.36 | −4.42 |
Bold values indicate significantly different levels of expression as determined by ANOVA. For the PM vs. WT in 0% and 10% v/v Populus hydrolysate, a positive/negative value represents a higher/lower expression level in the PM compared to the WT. For the standard medium (0%) versus Populus hydrolysate media (10 or 17.5%) positive/negative values represents higher/lower expression levels in the hydrolysate media compared to standard medium. Values are indicated for samples collected during mid-log (ML) and late-log (LL) growth phases.