Table 4.

Fold change in gene expression in histidine metabolism pathways

Gene	Product	PM vs. WT 0		PM vs. WT 10		PM 0 vs. 10		PM 0 vs. 17.5		WT 0 vs. 10
		ML	LL	ML	LL	ML	LL	ML	LL	ML	LL
Cthe_2880	ATP phosphoribosyltransferase regulatory subunit	98.42	29.12	98.72	80.51	1.25	1.01	1.12	−1.06	1.25	−2.73
Cthe_2881	ATP phosphoribosyltransferase	78.48	23.79	85.06	100.15	1.64	1.24	1.35	−1.01	1.52	−3.40
Cthe_2882	histidinol dehydrogenase	35.86	18.44	28.45	44.69	1.49	1.33	1.37	1.40	1.88	−1.83
Cthe_2883	histidinol-phosphate aminotransferase	38.12	19.61	23.12	40.22	1.15	1.22	1.19	1.42	1.89	−1.69
Cthe_2884	Imidazoleglycerol-phosphate dehydratase	7.45	7.71	17.31	17.09	1.23	1.25	1.18	1.27	−1.89	−1.77
Cthe_2886	Imidazole glycerol phosphate synthase subunit hisH	11.99	12.29	14.84	15.87	1.19	1.12	1.09	−1.01	−1.04	−1.16
Cthe_2887	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase	13.46	11.01	10.02	14.54	1.44	1.20	1.29	1.13	1.93	−1.10
Cthe_2888	Imidazole glycerol phosphate synthase subunit hisF	12.46	14.23	10.04	18.19	1.61	1.30	1.54	1.24	1.99	1.02
Cthe_2889	Histidine biosynthesis bifunctional protein hisIE	12.71	10.80	6.09	12.49	1.48	1.29	1.51	1.28	3.08	1.11
Cthe_3028	Pyridoxal-dependent decarboxylase	−11.35	−13.46	−7.10	−6.92	−2.37	−1.04	−3.78	−2.89	−3.79	−2.02
Cthe_3149	aminoacyl-histidine dipeptidase	3.34	4.23	−1.07	1.63	1.15	1.05	1.39	1.37	4.09	2.72
Cthe_1332	Histidyl-tRNA synthetase	−1.58	−1.89	1.66	−1.18	1.10	−1.03	−1.15	−1.62	−2.38	−1.64

Bold values indicate significantly different levels of expression as determined by ANOVA. For the PM vs. WT in 0% and 10% v/v Populus hydrolysate, a positive/negative value represents a higher/lower expression level in the PM compared to the WT. For the standard medium (0%) versus Populus hydrolysate media (10 or 17.5%) positive/negative values represents higher/lower expression levels in the hydrolysate media compared to standard medium. Values are indicated for samples collected during mid-log (ML) and late-log (LL) growth phases.