Table 5.
Fold change in gene expression along the cysteine and methionine metabolic pathway
|
Gene |
Product |
PM vs. WT 0 |
PM vs. WT 10 |
PM 0 vs. 10 |
PM 0 vs. 17.5 |
WT 0 vs. 10 |
|||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| ML | LL | ML | LL | ML | LL | ML | LL | ML | LL | ||
| Cthe_0290 |
homoserine dehydrogenase |
−1.03 |
1.21 |
2.33 |
1.94 |
−1.78 |
−1.38 |
1.35 |
1.17 |
1.45 |
−1.30 |
| Cthe_0580 |
aminotransferase class I and II |
1.22 |
1.48 |
−1.31 |
1.17 |
1.03 |
−1.00 |
1.44 |
2.03 |
1.64 |
1.26 |
| Cthe_0715 |
S-adenosylmethionine decarboxylase proenzyme |
1.21 |
1.33 |
2.95 |
−1.12 |
−1.51 |
−1.64 |
−1.87 |
−2.76 |
−3.67 |
−1.10 |
| Cthe_0755 |
aminotransferase class I and II |
−2.42 |
−1.28 |
1.59 |
−1.40 |
−1.75 |
−1.37 |
−1.60 |
−2.06 |
−6.77 |
−1.25 |
| Cthe_0961 |
aspartate-semialdehyde dehydrogenase |
−2.51 |
−2.11 |
−2.12 |
−1.37 |
1.18 |
1.15 |
1.47 |
2.34 |
−1.01 |
−1.34 |
| Cthe_1053 |
L-lactate dehydrogenase |
−1.78 |
−1.25 |
1.32 |
−1.02 |
−1.41 |
−1.27 |
−1.33 |
−1.16 |
−3.30 |
−1.55 |
| Cthe_1200 |
Adenosylhomocysteinase |
−1.26 |
1.07 |
2.23 |
1.76 |
1.39 |
1.18 |
1.01 |
−1.62 |
−2.02 |
−1.39 |
| Cthe_1559 |
Cys/Met metabolism pyridoxal-phosphate-dependent protein |
−9.22 |
−5.72 |
−4.73 |
−3.97 |
4.66 |
3.12 |
16.05 |
8.31 |
2.39 |
2.17 |
| Cthe_1560 |
Pyridoxal-5'-phosphate-dependent protein beta subunit |
−6.16 |
−2.97 |
−3.71 |
−2.65 |
6.25 |
3.41 |
15.55 |
6.43 |
3.77 |
3.05 |
| Cthe_1569 |
Cys/Met metabolism pyridoxal-phosphate-dependent protein |
1.02 |
1.09 |
−2.06 |
−1.83 |
3.94 |
2.46 |
5.21 |
4.42 |
8.24 |
4.90 |
| Cthe_1728 |
DNA-cytosine methyltransferase |
2.09 |
2.38 |
−1.21 |
2.26 |
1.03 |
−1.01 |
1.59 |
1.80 |
2.60 |
1.04 |
| Cthe_1749 |
DNA-cytosine methyltransferase |
1.08 |
−1.12 |
−5.98 |
−2.41 |
−1.08 |
1.20 |
1.13 |
1.46 |
5.95 |
2.58 |
| Cthe_1840 |
cysteine synthase A |
−1.52 |
−1.21 |
3.14 |
2.17 |
1.37 |
1.27 |
1.83 |
−1.27 |
−3.48 |
−2.07 |
| Cthe_1842 | O-acetylhomoserine/O-acetylserine sulfhydrylase | −1.68 | −1.54 | −1.10 | 1.52 | 1.51 | 1.16 | 2.46 | 1.75 | −1.02 | −2.01 |
Bold values indicate significantly different levels of express as determined by ANOVA. For the PM vs. WT in 0% and 10% v/v Populus hydrolysate, a positive/negative value represents a higher/lower expression level in the PM compared to the WT. For the standard medium (0%) versus Populus hydrolysate media (10 or 17.5%) positive/negative values represents higher/lower expression levels in the hydrolysate media compared to standard medium. Values are indicated for samples collected during mid-log (ML) and late-log (LL) growth phases.