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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Hepatology. 2013 Dec 24;59(2):651–660. doi: 10.1002/hep.26584

Fig. 2.

Fig. 2

Combined depletion of Cdk2 and CcnE1 results in strongly reduced CcnA2 expression and impaired DNA synthesis. Primary mouse hepatocytes from Cdk2f/f (WT), Cdk2Δhepa, and Cdk2ΔhepaCcnE1−/− mice were isolated and cultured for up to 3 days in the presence of EGF and insulin. (A) qPCR analysis of CcnA2 gene expression. (B) Down-regulation of CcnA2 in Cdk2ΔhepaCcnE1−/− mice at day 3 was confirmed by western blotting. (C) qPCR analysis of CcnE2 gene expression. (D) Determination of BrdU incorporation (green) in primary hepatocytes after 3 days in culture on coverslips. Total nuclei are counterstained with 4′, 6-diamidino-2-phenylindole (blue). (E) Quantification of BrdU-positive hepatocytes. *P < 0.05; ***P < 0.001.