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. 2014 Aug 1;14:206. doi: 10.1186/s12866-014-0206-6

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Copyright © 2014 Chen et al.; licensee BioMed Central Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Activation of promoters by BsaN/BicA inE. coli. The ability of BsaN and BicA to directly activate the expression of promoters was examined by providing regulatory genes in trans and measuring β-galactosidase activities arising from the expression of transcriptional promoter-lacZ fusions in E. coli DH5α. Effect of BsaN/BicA on the expression of A. PbicA-lacZ fusion, B. PbopA-lacZ fusion, C. PbopE-lacZ fusion, D. PbprD-lacZ fusion and E. Ps1518-lacZ fusions; Ps1518 denotes the promoter region of BPSS1518. Effect of BsaN/BicA on the expression of F. PvirA-lacZ fusion and G. PtssM-lacZ fusion. *p < 0.05.