Table 3.
List of strains used in this study
| Strain | Relevant characteristic(s) a | Source or reference |
|---|---|---|
|
E. coli
|
|
|
| DH5α |
lacZ− |
[25] |
| S17-1 |
Donor strain for conjugation |
[51] |
|
B. pseudomallei
|
|
|
| KHW |
Clinical strain |
[20] |
| ΔbsaN |
KHW, bsaN orf was deleted |
[14] |
| ΔbsaM |
KHW, bsaM orf was deleted |
[30] |
| ΔbopA |
KHW, bopA orf was replaced by zeocin resistance gene, Zeor |
[30] |
| ΔbopE |
KHW, bopE orf was deleted |
[30] |
| ΔbopC |
KHW, bopC orf was replaced by zeocin resistance gene, Zeor |
[30] |
| ΔbopACE |
KHW, bopA and bopE orfs were deleted, bopC orf was replaced by zeocin resistance gene, Zeor |
[30] |
| ΔBPSS1513-folE | KHW, BPSS1513 and folE orfs were deleted | This study |
aAbbreviations: Zeor zeocin resistant.