Table 4.
List of plasmids used in this study
| Plasmid | Relevant characteristic(s) a | Source or reference |
|---|---|---|
| pMLBAD |
Broad host range vector containing inducible promoter Pbad, Tmr |
[24] |
| pRW50 |
Low copy-number vector containing a promoter-less lacZ gene, Tcr |
[26] |
| pOT182 |
Source of oriT sequence, Tcr |
[27] |
| pK18mobsac |
Conjugative, suicide vector containing sacB gene, Kmr |
[52] |
| pRW50mob |
pRW50 containing oriT sequence from pOT182, Tcr |
This study |
| pMLbspR |
pMLBAD containing bspR orf from KHW, Tmr |
This study |
| pMLbprP |
pMLBAD containing bprP orf from KHW, Tmr |
This study |
| pMLbprQ |
pMLBAD containing bprQ orf from KHW, Tmr |
This study |
| pMLbprPQ |
pMLBAD containing bprP and bprQ orfs from KHW, Tmr |
This study |
| pMLbsaNs/bicA |
pMLBAD containing shorter bsaN orf (GTG start) and bicA orf from KHW, Tmr |
This study |
| pMLbsaN |
pMLBAD containing longer bsaN orf (ATG start) from KHW, Tmr |
This study |
| pMLbsaN/bicA |
pMLBAD containing longer bsaN orf (ATG start) and bicA orf from KHW, Tmr |
This study |
| pRWbsaM |
PbsaM-lacZ transcriptional fusion, pRW50mob containing bsaM upstream sequence from KHW, Tcr |
This study |
| pRWbsaN |
PbsaN-lacZ transcriptional fusion, pRW50mob containing bsaN upstream sequence from KHW, Tcr |
This study |
| pRWbicA |
PbicA-lacZ transcriptional fusion, pRW50mob containing bicA upstream sequence from KHW, Tcr |
This study |
| pRWbicA1 |
PbicA1-lacZ transcriptional fusion, pRW50mob containing truncated bicA upstream sequence from KHW, type 1 deletion, Tcr |
This study |
| pRWbicA2 |
PbicA2-lacZ transcriptional fusion, pRW50mob containing truncated bicA upstream sequence from KHW, type 2 deletion, Tcr |
This study |
| pRWbopE |
PbopE-lacZ transcriptional fusion, pRW50mob containing bopE upstream sequence from KHW, Tcr |
This study |
| pRWbopA |
PbopA-lacZ transcriptional fusion, pRW50mob containing bopA upstream sequence from KHW, Tcr |
This study |
| pRWbopA1 |
PbopA1-lacZ transcriptional fusion, pRW50mob containing truncated bopA upstream sequence from KHW, type 1 deletion, Tcr |
This study |
| pRWbopA2 |
PbopA2-lacZ transcriptional fusion, pRW50mob containing truncated bopA upstream sequence from KHW, type 2 deletion, Tcr |
This study |
| pRWbprB |
PbprC-lacZ transcriptional fusion, pRW50mob containing bprB upstream sequence from KHW, Tcr |
This study |
| pRWbprD |
PbprD-lacZ transcriptional fusion, pRW50mob containing bprD upstream sequence from KHW, Tcr |
This study |
| pRWbprD1 |
PbprD1-lacZ transcriptional fusion, pRW50mob containing truncated bprD upstream sequence from KHW, type 1 deletion, Tcr |
This study |
| pRWbprD2 |
PbicA2-lacZ transcriptional fusion, pRW50mob containing truncated bicA upstream sequence from KHW, type 2 deletion, Tcr |
This study |
| pRWbprC |
PbprC-lacZ transcriptional fusion, pRW50mob containing bprC upstream sequence from KHW, Tcr |
This study |
| pRWs1518 |
Ps1518-lacZ transcriptional fusion, pRW50 containing BPSS1518 upstream sequence from KHW, Tcr |
This study |
| pRWs1518a |
Ps1518a-lacZ transcriptional fusion, pRW50 containing truncated BPSS1518 upstream sequence from KHW, type 1 deletion, Tcr |
This study |
| pRWs1518b |
Ps1518b-lacZ transcriptional fusion, pRW50 containing truncated BPSS1518 upstream sequence from KHW, type 2 deletion, Tcr |
This study |
| pRWvirA |
PvirA-lacZ transcriptional fusion, pRW50 containing virA upstream sequence from KHW, Tcr |
This study |
| pRWvirA1 |
PvirA1-lacZ transcriptional fusion, pRW50 containing truncated virA upstream sequence from KHW, type 1 deletion, Tcr |
This study |
| pRWtssM | PtssM-lacZ transcriptional fusion, pRW50 containing tssM upstream sequence from KHW, Tcr | This study |
aAbbreviations: Apr ampicillin resistant, Kmr kanamycin resistant, Tcr tetracycline resistant, Tmr trimethoprim resistant.