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. 2014 Aug 7;14:204. doi: 10.1186/s12866-014-0204-8

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Copyright © 2014 Zheng et al.; licensee BioMed Central Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Se(IV) resistance and reduction using different Se(IV) concentrations using fouriscRinsertional mutants inC. testosteroniS44. The different sites of transposon insertions in iscR is given in nt from the translational start codon; +30 is an insertion upstream of iscS(A); the predicted domains of the IscR protein (B) and growth in LB medium amended with different concentrations of Se(IV) at different time points (C). The four arrows indicate the four mutants of iscR-280 (a), iscR-327 (b), iscR-513 (c) and iscS + 30 (d), respectively in A and B. The order of the 5 PA bottles of C is wild type (WT), iscR-280 (a), iscR-327 (b), iscR-513 (c) and iscS + 30 (d), respectively.