Table 6.
Real-time quantitative RT-PCR confirmation of selected genes
| Locus no. a | Primer sequence (5′-3′) b | Product size (bp) |
|---|---|---|
| 16S rRNA |
F: TGTTACAATGGGAGGGACAAAGGG |
118 |
| R: TTACTAGCGAATCCAGCTTCACGG | ||
| PG0090 |
F: CAGAAGTGAAGGAAGAGCACGAAC |
197 |
| R: GTAGGCAGACAGCATCCAAACG | ||
| PG0195 |
F: TCCACGGCTGAGAACTTGCG |
149 |
| R: TGCTCGGCTTCCACCTTTGC | ||
| PG1545 |
F: CCAAACCCTCAACCACAATC |
142 |
| R: GGTACCGGCTGTGTTGAACT | ||
| PG0593 |
F: CGTGTGGGAGAGTGGGTATTGG |
175 |
| R: CGCCGCTGTTGCCTGAATTG | ||
| PG1089 |
F: CCATCGCGATCGATGATCAGGTAA |
104 |
| R: GGCATAGTTGCGTTCAAGGGTTTC | ||
| PG1019 |
F: TTCGCAGTATCCCATCCAAC |
126 |
| R: TCCGGCTCATAGACTTCCAA | ||
| PG1180 |
F: CAGTCTGCCACAGTTCACCA |
124 |
| R: CCCTACACGGACACTACCGA | ||
| PG1983 |
F: GCTCTGTGGTGTGGGCTATC |
146 |
| R: GGATAACAGGCAAACCCGAT | ||
| PG0885 |
F: CAGATCCAAATCGGGACTGA |
156 |
| R: GTAGAGCAAGCCATGCAAGC | ||
| PG1181 | F: GATGAATTCGGGCGGATAAT |
184 |
| R: CCTTGAAGTGCTCCAACGAC |
aBased on the genome annotation provided by TIGR (http://cmr.jcvi.org/cgi-bin/CMR/GenomePage.cgi?org=gpg).
bPrimers were designed using Primer3 program for the study except for the primers of P. gingivalis 16S rRNA and PG1089 [49], which were prepared based on the primer sequences published previously. The 16S rRNA gene was used as the reference gene for normalization. F, forward; R, reverse.