Table 2.
Effect of phage on adhesion, invasion and cytotoxicity of NEC by additional clinical strains of S. aureus (MRSA)
| Strains (Bacteria: NEC- 10:1) |
Mean percent (%) |
||||||||
|---|---|---|---|---|---|---|---|---|---|
|
Adherence |
Invasion |
Cytotoxicity (24 h) |
|||||||
| No phage | Phage (MOI-1) | Phage (MOI-10) | No phage | Phage (MOI-1) | Phage (MOI-10) | No phage | Phage (MOI-1) | Phage (MOI-10) | |
|
S. aureus ATCC 43300 (MRSA) |
73.7 |
0.41 |
0.025 |
31.9 |
0.031 |
No invasion |
11.1 |
0.21 |
No cytotoxicity |
|
S. aureus ATCC 29213 (MSSA) |
76.8 |
0.51 |
0.034 |
18.4 |
0.034 |
No invasion |
10.2 |
0.23 |
No cytotoxicity |
|
S. aureus CS-1 |
68.4 |
0.37 |
0.066 |
28.1 |
0.06 |
No invasion |
11.4 |
0.41 |
No cytotoxicity |
|
S. aureus CS-2 |
62.5 |
0.32 |
0.074 |
25.4 |
0.064 |
No invasion |
10.1 |
0.43 |
No cytotoxicity |
|
S. aureus CS-3 |
74.8 |
0.45 |
0.084 |
33.3 |
0.078 |
No invasion |
14.5 |
0.64 |
No cytotoxicity |
|
S. aureus CS-4 |
70.4 |
0.34 |
0.081 |
30.4 |
0.072 |
No invasion |
14 |
0.61 |
No cytotoxicity |
| S. aureus CS-5 | 72.1 | 0.33 | 0.075 | 32.8 | 0.066 | No invasion | 13.3 | 0.72 | No cytotoxicity |
(CS-1 to CS-5 : these are clinical strains (CS) of MRSA chosen at random to test for their adherence, invasion and cytotoxicity parameters on cultured murine NEC).