Table 1.
Region | Allele | Primer name | Primer sequence |
---|---|---|---|
Proximal | Rye |
o-sec-upp a o-sec-low |
5′ TCA AGG TGT GTA GTG TAA AG 3′ 5′ GAC TTC ACT TCA TAA ATA GG 3′ |
Proximal | Wheat |
wPt-1911 F1b wPt-1911 R1 |
5′ GAG GTG TAC CGA TGG CTG TT 3′ 5′ GCA GTT GTG TAG CCG TTG TG 3′ |
Distal | Wheat |
psp3000 F1c psp3000 R1 |
5′ GCA GAC CTG TGT CAT TGG TC 3′ 5′ GAT ATA GTG GCA GCA GGA TAC G 3′ |
Distal | Wheat/rye |
gpw7059 F1d gpw7059 R1 |
5′ AAC ACC AAT GAC CTG ATC GC 3′ 5′ TCC TCA ACA GCT CCA GTG C 3′ |
a Shimizu et al. (1997)
b Diversity arrays technologies (www.diversityarrays.com)
c Devos et al. (1995)
d Sourdille and Bernard (unpublished data, http://wheat.pw.usda.gov/cgi-bin/graingenes/report.cgi?class=marker&name=GPW7059)