Table 2.

Experimental field design

Location	Year	Sowing date	Harvest date	Fertilizationa N	Genotypesb	Design and plot sizec	Rep.d (irr)	Traitse
Davis	08–09	10/30/08	6/11/09	67.25 kg/ha pre-plant and 2 × 44.8 kg/ha	H, H1RSWW, A, A1RSWW	RCBD split-plot 4.5 m2	10 (L)	Yield
Davis	10–11	11/09/10	7/5/11	67.25 kg/ha pre-plant and 2 × 44.8 kg/ha	H, H1RSWW, A, A1RSWW	RCBD split–split-plot 4.5 m2	6 (N × 2) 6 (L × 2)	Yield, CSR, CID
Davis	11–12	10/27/11	6/7/12	67.25 kg/ha pre-plant and 2 × 44.8 kg/ha	H, H1RSWW, H1RSWR, H1RSRW	RCBD 2 × 1 m row	20 (N)	CSR
Davis	12–13	11/6/12	6/5/13	67.25 kg/ha pre-plant and 2 × 44.8 kg/ha	H, H1RSWW, H1RSWR, H1RSRW	RCBD 4.5 m2	10 (N) 10(L)	Yield, CSR, GE, CID
DREC	09–10	12/12/09	5/20/10	112 kg/ha pre-plant and 135, 86 and 24 kg/ha	H, H1RSWW, A, A1RSWW	RCBD 2 × 1 m row	6 (N)	Yield, CID
DREC	10–11	12/15/10	5/5/11	134 kg/ha pre-plant and 136,100 kg/ha	H, H1RSWW, A, A1RSWW	RCBD split–split-plot 7.4 m2	6 (N) 6 (L)	Yield

^a N was applied as ammonium sulfate in Davis and anhydrous ammonia in DREC

^b H Hahn (1RS), A Attila (1RS)

^c In the split-plot designs, cultivar was used as the main plot factor and genotype (original 1RS vs 1RS^WW) was used as the subplot factor. In split–split-plot designs, irrigation treatment was the main plot factor, cultivar was the subplot factor and genotype was the sub–subplot factor

^d N normal irrigation, L without final irrigation

^e CSR canopy spectral reflectance, CID carbon isotope discrimination, GE gas exchange