Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov 19;4:7111. doi: 10.1038/srep07111

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2014, Macmillan Publishers Limited. All rights reserved

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

PMC Copyright notice

(a) Low intensity (2% of maximal laser power) TIRF illumination was applied during both the pre-bleach (top) and post-bleach-recovery phase (bottom). During the bleaching phase, the TIRF angle was maintained, but laser power was increased to 100%. (b,c) Epifluorescence and TIRF micrographs of TRPV2-GFP and TRPM4-GFP transfected in HEK293 cells before and immediately following bleaching. (d,e) Fluorescence intensities in epifluorescence and TIRF normalized to the respective pre-bleach intensities.