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. 2014 Aug 5;14:208. doi: 10.1186/s12870-014-0208-4

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Copyright © 2014 Xu et al.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Molecular and phenotypic analysis of SA transgenic cassava coupled expression of cytosolicMeCu/ZnSOD and cytosolicMeAPX2 genes. (a) Schematic presentation of the T-DNA region of pC-P54::MeCu/ZnSOD-35S::MeAPX2 with unique XbaI site. LB, left border; RB, right border of T-DNA; T_35S, CaMV 35S terminator; T_NOS, NOS terminator ; P_35S, cauliflower mosaic virus 35S promoter; P_54/1.0, vascular-specific promoter p54/1.0; HPT, hygromycin phosphotransferase. (b) Southern blot analysis of transgenic and WT cassava plants for transgene integration. Transgene integration patterns in SA lines detected XbaI-digested genomic DNA by HPT (left panel) and MeAPX2 (right panel) probes. gAPX, genomic DNA of cassava ascorbate peroxidase; T-APX2, transgene MeAPX2; M, λ HindIII DIG-labeled molecular marker; P, plasmid pC-P54::MeCu/ZnSOD-35S::MeAPX2; WT, wild-type control. Numbers indicate different transgenic lines. (c) Plant growth status and phenotype evaluation in field. WT, wild-type control; SA1, SA2 and SA4, independent transgenic cassava plant lines.