Table 4.
Antibodies used to detect the cell surface markers
| Target | Antibody | Company |
|---|---|---|
| CD79a |
PE-conjugated mouse anti-bovine CD79a |
Abcam, UK |
| CD3 |
FITC-conjugated hamster anti-bovine CD3 |
Abcam |
| CD4 |
PE-conjugated mouse anti-bovine CD4 |
US Biological, USA |
| CD8 |
PE-conjugated mouse anti-bovine CD8 |
LifeSpan BioSciences, USA |
| MHC Ι |
FITC-conjugated mouse anti-bovine MHC Ι |
Novus Biological, USA |
| MHC ΙΙ |
Mouse anti-bovine MHC ΙΙa |
Abcam |
| Fc receptor blocking | Polyclonal mouse IgG fraction |
Abcam |
| Monoclonal Armenian hamster IgG fraction | Abcam |
aMouse anti-bovine MHC ΙΙ antibody was conjugated with PE using an EasyLink B-Phycoerythrin conjugation kit (Abcam).