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Schematic representation of pNZ8148-SPUsp45-MpEGF vector expression of MpEGF inL. lactis. A 159-bp DNA fragment encoding mature porcine EGF (MpEGF) was fused in frame with an 81-bp DNA fragment containing the Usp45 signal peptide, derived from the predominant L. lactis-secreted protein. In this plasmid, pEGF expression is controlled by the nisin-inducible promoter (PnisA). The pNZ8148-SPUsp45-MpEGF carries the chloramphenicol resistance marker (cm).
