Figure 2.
miR-140-5p controls the ErbB4 expression. ErbB4 expression in esophageal cancer cell lines (ECA 109, EC9706, and TE-1) and normal cell line (HET-1A) via western blot analysis (a). miR-140-5p expression in these cell lines (b). Western blot analysis of effect of miR-140-5p on ErbB4 protein expression (c and d). RT-PCR analysis of effect of miR-140-5p on ErbB4 mRNA expression (e). Analysis of luciferase reporter activity in EC9706 cancer cell line (f). The luciferase activity was examined by Renilla luciferase method.