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. Author manuscript; available in PMC: 2014 Nov 19.
Published in final edited form as: Methods Mol Biol. 2014;1172:209–218. doi: 10.1007/978-1-4939-0928-5_19

Table 1.

Example: for 3 wells of a 12-well dish, we multiply by a 10 % factor, or 1.1 to account for pipetting errors

Vector Concentration (µg/µL) Tube 1 Tube 2
DNA: (0.5 µg/well) × 3 wells × 1.1 (µL) DNA dilution: Serum-free medium (100 µL per well × 3 wells × 1.1) (100 × 3 × 1.1) − (DNA)= Lipofectamine 2000: 2.0 µL per well × 3 wells × 1.1 (µL) Lipo dilution: Serum-free medium (100 µL per well × 3 wells × 1.1) (100 × 3 × 1.1)− (Lipo)=
Control 0.588 (0.5 × 3 × 1.1)/0.588 = 2.81 327.19 (2 × 3 × 1.1) = 6.6 323.4
shCXCR7 0.441 (0.5 × 3 × 1.1)/0.441 = 3.74 326.26 (2 × 3 × 1.1) = 6.6 323.4