Table 1. Studies to Assess HIV-1 Reservoirs after Allogeneic Stem Cell Transplantation and Before Antiretroviral Treatment Interruption.
Sample Type | Quantity | Total Cells Tested | No. of Positive Replicates/ No. of Wells |
---|---|---|---|
Patient A (4.3 years post-HSCT) | |||
Total HIV-1 PBMC DNA | <0.12 copies/106 cells | 26×106 PBMCs | 0/42 |
Infectious Virus by Co-Culture | <0.007 IUPMa | 150×106 CD4+ T Cells | 0/30 |
Patient B (2.6 years post-HSCT) | |||
Total HIV-1 PBMC DNA | <0.13 copies/106 cells | 24×106 PBMCs | 0/42 |
Infectious Virus by Co-Culture | <0.006 IUPMa | 160×106 CD4+ T Cells | 0/32 |
Rectal Tissue | <2.4 copies/106 cells | 1.23×106 nucleated cellsb | 0/22 |
Abbreviations: HIV, human immunodeficiency virus; HSCT, hematopoietic stem cell transplantation; PBMC, peripheral blood mononuclear cell; IUPM, infectious units per million cells
No replication-competent HIV-1 was recovered
25% CD4 + T cells determined by flow cytometry after tissue disaggregation and cell separation by density centrifugation