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. Author manuscript; available in PMC: 2015 Sep 2.
Published in final edited form as: Ann Intern Med. 2014 Sep 2;161(5):319–327. doi: 10.7326/M14-1027

Table 1. Studies to Assess HIV-1 Reservoirs after Allogeneic Stem Cell Transplantation and Before Antiretroviral Treatment Interruption.

Sample Type Quantity Total Cells Tested No. of Positive Replicates/ No. of Wells
Patient A (4.3 years post-HSCT)
 Total HIV-1 PBMC DNA <0.12 copies/106 cells 26×106 PBMCs 0/42
 Infectious Virus by Co-Culture <0.007 IUPMa 150×106 CD4+ T Cells 0/30
Patient B (2.6 years post-HSCT)
 Total HIV-1 PBMC DNA <0.13 copies/106 cells 24×106 PBMCs 0/42
 Infectious Virus by Co-Culture <0.006 IUPMa 160×106 CD4+ T Cells 0/32
 Rectal Tissue <2.4 copies/106 cells 1.23×106 nucleated cellsb 0/22

Abbreviations: HIV, human immunodeficiency virus; HSCT, hematopoietic stem cell transplantation; PBMC, peripheral blood mononuclear cell; IUPM, infectious units per million cells

a

No replication-competent HIV-1 was recovered

b

25% CD4 + T cells determined by flow cytometry after tissue disaggregation and cell separation by density centrifugation