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. 2014 Nov 19;5:622. doi: 10.3389/fpls.2014.00622

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Copyright © 2014 Yu and Pilot.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of the relationship between amiRNA expression, target mRNA and protein accumulations. Two amiRNAs targeting (A) LOG2 and (B) GDU1. Leaves of N. benthamiana were infiltrated with various ratios of amiRNA / target gene (mLOG2-HA or GDU1-HA), ranging from 0.1 to 4. Control (ratio 0, left panels) corresponds to co-expression of the target gene with SUC2. The ratio 0 for the right panels corresponds to co-expression of the target genes with amiRNA^SnRK1.1-C. The same amount of the target constructs was infiltrated for each sample. Variable amounts of amiRNA^GDU1 (or amiRNA^LOG2) and amiRNA^SnRK1.1-C were co-infiltrated to keep constant the total amount of amiRNA expressed in the leaf. Target mRNA and protein levels were determined by qRT-PCR and western blot respectively. Error bars represent standard error processed by qbase^PLUS. Levels are expressed relative to the 0 ratio of the left panels.