Fig. 3.

Immunostaining showing expression of 25-hydroxyvitamin D 1α-hydroxylase by the metastatic tumor. Panel A, Darkfield microscopy showing expression of 25-hydroxyvitamin D 1α-hydroxylase in tumor cells (center of section), but not in the adjacent normal liver parenchyma (bottom right) (original magnification ×100). Panel B, Corresponding hematoxylin and eosin stain showing liver tissue infiltrated by a malignant neoplasm composed of cells with clear and eosinophilic cytoplasm (original magnification ×100). Panel C, Expression of 25-hydroxyvitamin D 1α-hydroxylase by renal tubular epithelial cells (positive control) (original magnification ×100). Panel D, Hematoxylin and eosin stain of section corresponding to Panel C (original magnification ×100).