Figure 4.

Effects of ampelopsin on c-Myc and Skp2 expression levels in A549 human pulmonary adenocarcinoma cells. (A) The relative c-Myc and Skp2 mRNA levels were detected using quantitative polymerase chain reaction with GAPDH as an internal control. The results are expressed as the mean ± standard error of the mean from five independent experiments. (B) c-Myc and Skp2 protein levels were assayed by western blotting. β-actin served as an internal control. In these experiments, the A549 cells were exposed to 0, 10, 20 and 30 μM ampelopsin for 48 h. ^*P<0.05 and ^**P<0.01 vs. the control group. Skp2, S-phase kinase-associated protein 2.