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. 2014 Oct 21;11(1):105–112. doi: 10.3892/mmr.2014.2733

Figure 5.

Figure 5

Effects of ampelopsin on Fbw7 and GSK3β expression levels in A549 human pulmonary adenocarcinoma cells. (A) Relative Fbw7α, Fbw7β, Fbw7γ and GSK3β mRNA levels were detected using quantitative polymerase chain reaction with GAPDH as an internal control. The results are expressed as the mean ± standard error of the mean from five independent experiments. (B) Fbw7α, Fbw7β, Fbw7γ, Thr58-Myc and GSK3β protein levels were assayed by western blotting. β-actin served as an internal control.*P<0.05 and **P<0.01 vs. the control group. Fbw7, F-box and WD repeat-containing protein 7; GSK3β, glycogen synthase kinase 3β; p−, phosphorylated−.