Table I.
Primer sequences for quantitative polymerase chain reaction.
| Gene | Orientation | Primer sequence (5′-3′) |
|---|---|---|
| GAPDH | Forward | GAAATCCCATCACCATCTTCCAGG |
| Reverse | GAGCCCCAGCCTTCTCCATG | |
| HDAC1 | Forward | TAAATTCTTGCGCTCCATCC |
| Reverse | AACAGGCCATCGAATACTGG | |
| HDAC2 | Forward | CGTGTAATGACGGTATCATTCC |
| Reverse | ACCAGATAATGAGTCTGCACC | |
| c-Myc | Forward | AGCGACTCTGAGGAGGAACAAG |
| Reverse | GTGGCACCTCTTGAGGACCA | |
| Skp2 | Forward | TGGGAATCTTTTCCTGTCTG |
| Reverse | GAACACTGAGACAGTATGCC | |
| Fbw7α | Forward | AGTAGTATTGTGGACCTGCCCGTT |
| Reverse | GACCTCAGAACCATGGTCCAACTT | |
| GSK3β | Forward | GGCAGCATGAAAGTTAGCAGA |
| Reverse | GGCGACCAGTTCTCCTGAATC |
HDAC, histone deacetylase; Skp2, S-phase kinase-associated protein 2; Fbw7α, F-box and WD repeat-containing protein 7α; GSK3β, glycogen synthase kinase 3β.