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LAC increased cisplatin-induced ER stress in HeLa human cervical cancer cells. (A) HeLa cells were treated with cisplatin (5 μg/ml) and/or LAC (10 μM) for 12 h. Western blot analysis of Ub, PDI and p62. (B) Quantification of the protein levels. Data are presented as the mean ± standard deviation, n=3. *P<0.05 vs. control, #P<0.05 vs. cisplatin. LAC, lactacystin; ER, endoplasmic reticulum; Ub, ubiquitinated protein; PDI, protein disulfide isomerase.
