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MLT decreases MLCK activity in the artery wall. MLCK activity was measured by rates of γ-32P-adenosine triphosphate incorporation into MLC as the substrate. *P<0.05 vs. control; #P<0.05 vs. AS group. Each assay was repeated three times. Con, control; AS, atherosclerosis model group; MLT, melatonin-treated AS group; MLCK, myosin light chain kinase.
