Figure 4.

Effects of 3-methyladenine (3-MA) and Beclin 1 small interfering (si)RNA on the apoptosis of A2780cp ovarian cancer cisplatin-resistant cells. (A) The apoptotic rate of cells treated in the absence, or presence, of cisplatin and 3-MA was determined. (B) The apoptotic rate of cells treated in the absence or presence of cisplatin and Beclin 1 siRNA transfection was determined. The data are represented as the means ± standard deviation, of three independent experiments. ^**P< 0.01 (C) The cells were treated with or without cisplatin (6 μg/ml) and 3-MA (1 mmol) for 24 h. (D) The cells were transfected with Beclin 1 siRNA (100 nM) for 48 h, and treated with or without cisplatin for 24 h. All of the apoptotic levels were determined using the fluorescien isothiocyanate (FITC) Annexin V apoptosis assay. PI, propidium iodide.