Figure 1.

Lentiviral vector and transduced mouse and human gut-derived cells. (A) Map showing the lentiviral plasmid construct that expresses either eGFP or mCherry under the spleen focus forming virus (SFFV) promoter and the mutated Woodchuck Posttranscriptional Regulatory Element (WPRE) which enhances transgene expression and titer. (B) Representative gating for FACS analysis of lentivirally transduced (eGFP) mouse or human gut-derived cells showing separation of transduced vs non-transduced cells. (C and D) Transduced cells highly express the eGFP reporter gene for prolonged time in culture (C, mouse ENS cells after 65 days and D, human ENS cells after 35 days). (E and F) Transduced cells form characteristic neurospheres similar in appearance to previously reported non-transduced cells.¹⁰ Scale bars = 50 μm (C and D) and 100 μm (E and F).