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. 2013 Aug 30;51(11):763–776. doi: 10.1002/dvg.22423

Figure 3.

Figure 3

Contribution of iPSCs to the ICM of blastocysts. (a) Porcine iPSCs (Porco Rosso-4 and Porco Rosso-622-14) were aggregated with porcine parthenogenetic 4- to 8-cell-, or morula-stage embryos. After in vitro culture, contribution of iPSCs to blastocysts was analyzed by fluorescence of the transgene, humanized Kusabira-Orange (huKO). Scale bar = 50 µm. (b) The percentage of ICM contribution of Porco Rosso-4 and Porco Rosso-622-14. Aggregation experiments using porcine iPSCs (Porco Rosso-4 and Porco Rosso-622-14) were performed twice independently (Exps. 1 and 2). (c) Summary of the contribution of iPSCs to the ICM of blastocysts. Using the ICM cells dissociated from blastocysts as donor cells, most aggregated embryos developed into blastocysts, and donor ICM cells could contribute efficiently to the ICM of the host blastocyst, confirming the contribution of pluripotent cells to the ICM. Statistical comparison was performed by chi-square test.