Figure 5.

Effect of V-ATPase inhibitor on Rickettsia montanensis infection of DVE1 cells. DVE1 cells were treated for 2 h with bafilomycin A1 (BAF) prior to infection with R. montanensis at a multiplicity of infection of 10. After 1 h, Rickettsia was removed. The cells were washed twice with phosphate-buffered saline and collected by low-speed centrifugation. Genomic DNA was then isolated and percent relative invasion was assessed by quantitative PCR. Data shown are mean percent relative invasion from two independent experiments. Error bar represents sem values. The asterisks mark significant difference from untreated control cells (*P = 0.0005).