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. Author manuscript; available in PMC: 2015 Sep 29.
Published in final edited form as: Dev Cell. 2014 Sep 29;30(6):717–730. doi: 10.1016/j.devcel.2014.08.003

Figure 4.

Figure 4

Both CENP-A chromatin stretch and CENP-T stretch contribute to intrakinetochore stretch. Kinetochore immunofluorescence of both GFP and Ndc80/Hec1(9G3) or GFP and CENP-A in GFP-CENP-T or CENP-T-GFP stably expressed cell (A) treated with CENP-C RNAi (B) or CENP-C/-H RNAi (C). Corresponding Delta analysis (right). (D) Summary of mean separation measurements between CENP-A and C-terminus of CENP-T and the C-terminus of CENP-T to Spc24/Spc25 for changes in intrakinetochore stretch between normal late prometaphase and metaphase, and for changes in late prometaphase induced by depletion of CENP-C and CENP-C/-H.