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. Author manuscript; available in PMC: 2015 Sep 1.
Published in final edited form as: Autoimmunity. 2014 May 6;47(6):409–418. doi: 10.3109/08916934.2014.914507

Figure 4. MTX and 1MT do not synergize to further inhibit autoantibody production.

Figure 4

K/BxN mice were treated with Carrier, MTX alone (1mg/kg), 1MT alone (400mg/kg), or MTX + 1MT from 3–6wk of age. (A) Serum anti-GPI Ig titers were measured using an ELISA assay. The graph shows the mean titer ± SEM from n=16 Carrier, n=10 1MT, n=14 MTX, and n=14 MTX+1MT treated mice, pooled from 3 independent experiments. (B) Antibody Secreting Cells (ASCs) from the joint draining LN were measured using an ELISpot assay. The graph represents the mean number of ASCs ± SEM from n=13 Carrier, n=10 1MT, n=18 MTX, and n=14 MTX+1MT treated mice, pooled from 3 independent experiments. *p<0.05, **p<0.01, n.s., not significant.