Table 1. MTX and 1MT do not synergize to inhibit inflammatory cytokine production.

K/BxN mice were treated with Carrier, MTX alone (1mg/kg), 1MT alone (400mg/kg), or MTX + 1MT from 3–6wk of age. At 6wk, cells from the joint dLNs (popliteal, axillary, and brachial LNs) were harvested and cultured overnight in media alone or PMA (50ng/ml) + ionomycin (500ng/ml). Cytokines were measured in culture supernatants by cytometric bead array. Table shows the mean concentration ± SEM from n=20 Carrier, n=13 1MT, n=14 MTX, and n=12 MTX+1MT treated mice, pooled from 3 independent experiments.

Cytokine (pg/ml)	Carrier	1MT	MTX	MTX+1MT
IL-4	2 ± 1	0 ± 0	5 ± 3	4 ± 3
IL-5	77 ± 9	46 ± 7*	117 ± 24	102 ± 13
IL-6	43 ± 3	27 ± 5*	39 ± 2	37 ± 5
IL-9	20 ± 4	21 ± 5	16 ± 5	20 ± 7
IL-10	144 ± 22	75 ± 17*	51 ± 15*	59 ± 19*
IL-13	109 ± 12	87 ± 18	174 ± 35	154 ± 22
IL-17	895 ± 140	709 ± 284	1061 ± 189	1226 ± 267
IFNγ	6107 ± 370	4526 ± 727*	5731 ± 650	5999 ± 871
MCP-1	159 ± 13	104 ± 13*	156 ± 19	144 ± 17
MIP-1α	662 ± 16	650 ± 55	655 ± 17	630 ± 16
MIP-1β	160 ± 6	175 ± 15	161 ± 6	153 ± 5
RANTES	616 ± 38	551 ± 89	745 ± 37	629 ± 45
TNFα	391 ± 35	224 ± 55*	371 ± 29	364 ± 42

^*p<0.05