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. Author manuscript; available in PMC: 2014 Nov 19.
Published in final edited form as: Org Lett. 2010 May 21;12(10):2330–2333. doi: 10.1021/ol100684s

Figure 3.

Figure 3

PAGE analysis of DEBS ACP3 (apo and holo), ACP2 (apo and holo), KS-AT3 and KS-AT6 reaction with compound 1 and subsequent click reaction with rhodamine-azide. Lanes are marked above with the corresponding protein component. Markers to the left indicate expected bands for the indicated species. The right two lanes depict transfer of the acylation product from preloaded ACP to KSAT indicating that the intermediates are functional. β-lactone was applied at 10× relative to protein unless otherwise noted. ACP2 contains a C-terminal linker region accounting for its larger mass.