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. 2014 Nov 18;5:5342. doi: 10.1038/ncomms6342

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Copyright © 2014, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.

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Infection of Vero cells by live recombinant NiV expressing secreted Gaussia luciferase (rNiV-GLuc, MOI=1) in the presence of 1:100 dilution of the indicated sera: FCS (fetal calf serum, negative control), NHS (normal human serum, negative control), rabbit anti-NiV (positive control), two seronegative samples (picked randomly among the 480 seronegative samples) and four seropositive samples. 20 μl (out of 150 μl) of infected cell culture supernatant was collected and analysed for Gaussia luciferase activity at 24 h.p.i. Infectivity data are presented as mean relative light units (RLUs)±s.d. from three independent replicates. Significant differences (asterisk) were observed between seropositive and seronegative sera (P<0.05; one-tailed Student’s t-test followed by the Holm step-down procedure for multiple comparisons).

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