Figure 3.
Tenovin-6 induces p21 expression without affecting p53 acetylation at lysine 382. (a) Expression of p53 and acetylated p53 (Ac 382Kp53). Cells were incubated with 2 μM Tv6, and total protein extracts from the cells were collected after 6 and 24 h for western blot using specific antibodies and infrared conjugates (IR-dye conjugates), and visualized simultaneously using the Odyssey system. (b) CDKN1A/p21 expression in Tv6-treated and control cells determined by QRT-PCR. Relative expression in relation to GAPDH is shown