Table 1. Genetic markers, p53 status and histology of soft tissue sarcoma cell lines.
| Sarcoma subtype | Cell line | Genetic arrangement | P53gene status | Tv6 IC50 |
|---|---|---|---|---|
| SS | K-SS1 | SS18/SSX1 | wt | 2.7 |
| SYO-1 | SS18/SSX2 | wt | 5.5 | |
| BAX | SS18/SSX2 | wt | 3.6 | |
| 1273/99* | SS18/SSX2 | wt | 4 | |
| RMS | RMS (a) | PAX3/7-FOXO1 | Mut | 2.3 |
| RH30 (a) | PAX3/7-FOXO1 | Mut | 1 | |
| RD (e) | 22q- | Mut | 3.5 |
Abbreviations: RMS, rhabdomyosarcomas; SS, synovial sarcomas. Alveolar rhabdomyosarcoma (a); embryonal rhabdomyosarcoma (e) and SV-40 transformed (*). The fusion gene type and the status of the p53 gene was determined using RT-PCR and dye terminator sequencing. IC50 were determined by exposing the cells to different concentrations of tenovin-6 and determining viability after 48 h using the Wst-1 assay (Supplementary Figure 1).